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Production of thermally induced recombinant proteins relative to cell biomass is influenced by cell density in Escherichia coli batch cultures

José Luis Corchero1, Pau Vila1, Rafael Cubarsi2 and Antonio Villaverde1

(1) Institut de Biologia Fonamental and Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
(2) Departament de Matemàtica Aplicada i Telemàtica, Universitat Politècnica de Catalunya, 08034, Barcelona, Spain

Summary  Fed-batch cultures of recombinant Escherichia coli were studied regarding their ability to permit CI857ts-controlled gene expression at different growth phases. Cells, growing at 50% pO2 and 28°C in a bioreactor, were transferred to a shaker flask at 42°C and the product yield was analysed after a short incubation time. Within the batch period, the synthesis of recombinant beta-galactosidase relative to the increase of biomass during the induction period was enhanced in an biomass-dependent fashion. Whereas in young cells biomass production dominates over recombinant protein production, the available energetic resources of aged cells seem to be employed more efficiently for CI857-controlled biosynthesis of recombinant proteins than of cell material. A model to describe this lsquoaltruisticrsquo behaviour of old cultures is here proposed.

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